Consensus-based evaluation of RIDASCREEN TB ELISA compared with two interferon-gamma release assays in a low-incidence setting

WHO Global Tuberculosis Report (2025) n.d https://www.who.int/teams/global-programme-on-tuberculosis-and-lung-health/tb-reports/global-tuberculosis-report-2025 (accessed April 15, 2026)

Sciensano (2026) accessed April 15,. Communicable Diseases: Tuberculosis, Health Status Report, 13 June 2025, Brussels, Belgium, n.d. https://www.healthybelgium.be/en/health-status/communicable-diseases/tuberculosis

Sossen B, Richards AS, Heinsohn T, Frascella B, Balzarini F, Oradini-Alacreu A et al (2023) The natural history of untreated pulmonary tuberculosis in adults: a systematic review and meta-analysis. Lancet Respir Med 11:367–379. https://doi.org/10.1016/S2213-2600(23)00097-8

Article  PubMed  Google Scholar 

Shah M, Dorman SE (2021) Latent Tuberculosis Infection. N Engl J Med 38:13–14. https://doi.org/10.1056/NEJMCP2108501

Article  Google Scholar 

Gill CM, Dolan L, Piggott LM, McLaughlin AM New developments in tuberculosis diagnosis and treatment. Breathe 2022;18. https://doi.org/10.1183/20734735.0149-2021

Pai M, Denkinger CM, Kik SV, Rangaka MX, Zwerling A, Oxlade O et al (2014) Gamma Interferon Release Assays for Detection of Mycobacterium tuberculosis Infection. Clin Microbiol Rev 27:3–20. https://doi.org/10.1128/CMR.00034-13

Article  CAS  PubMed  PubMed Central  Google Scholar 

Goletti D, Delogu G, Matteelli A, Migliori GB (2022) The role of IGRA in the diagnosis of tuberculosis infection, differentiating from active tuberculosis, and decision making for initiating treatment or preventive therapy of tuberculosis infection. Int J Infect Dis 124:S12–S19. https://doi.org/10.1016/J.IJID.2022.02.047

Article  CAS  PubMed  Google Scholar 

Zhou G, Luo Q, Luo S, Chen H, Cai S, Guo X et al (2023) Indeterminate results of interferon gamma release assays in the screening of latent tuberculosis infection: a systematic review and meta-analysis. Front Immunol 14:1170579. https://doi.org/10.3389/FIMMU.2023.1170579/BIBTEX

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen H, Nakagawa A, Takamori M, Abe S, Ueno D, Horita N et al (2022) Diagnostic accuracy of the interferon-gamma release assay in acquired immunodeficiency syndrome patients with suspected tuberculosis infection: a meta-analysis. Infection 50:597–606. https://doi.org/10.1007/S15010-022-01789-9/FIGURES/4

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ortiz-Brizuela E, Apriani L, Mukherjee T, Lachapelle-Chisholm S, Miedy M, Lan Z et al (2023) Assessing the Diagnostic Performance of New Commercial Interferon-γ Release Assays for Mycobacterium tuberculosis Infection: A Systematic Review and Meta-Analysis. Clin Infect Dis 76:1989–1999. https://doi.org/10.1093/CID/CIAD030

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kumar NP, Hissar S, Thiruvengadam K, Banurekha VV, Balaji S, Elilarasi S et al (2021) Plasma chemokines as immune biomarkers for diagnosis of pediatric tuberculosis. BMC Infect Dis 21:1055. https://doi.org/10.1186/S12879-021-06749-6

Article  CAS  PubMed  PubMed Central  Google Scholar 

Daniel EA, Thiruvengadam K, Rajamanickam A, Chandrasekaran P, Pattabiraman S, Bhanu B et al (2023) QuantiFERON Supernatant-Based Host Biomarkers Predicting Progression to Active Tuberculosis Disease Among Household Contacts of Tuberculosis Patients. Clin Infect Dis 76:1802–1813. https://doi.org/10.1093/CID/CIAC979

Article  CAS  PubMed  Google Scholar 

Kim S, Kim YK, Lee H, Cho JE, Kim HY, Uh Y et al (2013) Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay. Diagn Microbiol Infect Dis 75:68–72. https://doi.org/10.1016/J.DIAGMICROBIO.2012.09.015

Article  CAS  PubMed  Google Scholar 

Sivakumaran D, Ritz C, Gjøen JE, Vaz M, Selvam S, Ottenhoff THM et al (2021) Host Blood RNA Transcript and Protein Signatures for Sputum-Independent Diagnostics of Tuberculosis in Adults. Front Immunol 11:626049. https://doi.org/10.3389/FIMMU.2020.626049/BIBTEX

Article  PubMed  PubMed Central  Google Scholar 

Strzelak A, Komorowska-Piotrowska A, Borowa A, Krasińska M, Feleszko W, Kulus M (2024) IP-10 for the Diagnosis and Treatment Monitoring of Tuberculosis in Children. Diagnostics 2024, Vol 14, Page 177. ;14:177. https://doi.org/10.3390/DIAGNOSTICS14020177

Blauenfeldt T, Villar-Hernández R, García-García E, Latorre I, Holm LL, Muriel-Moreno B et al (2020) Diagnostic Accuracy of Interferon Gamma-Induced Protein 10 mRNA Release Assay for Tuberculosis. J Clin Microbiol 58. https://doi.org/10.1128/JCM.00848-20

Iqhrammullah M, Yusnaini R, Amirah S, Mulya IC, Tsurayya G, Naufal MA et al (2024) Effect of tuberculosis-specific antigen stimulation on the diagnostic accuracy of interferon-γ inducible protein-10 in distinguishing active and latent tuberculosis infection: a meta-analysis. Microbes Infect 26:105396. https://doi.org/10.1016/J.MICINF.2024.105396

Article  CAS  PubMed  Google Scholar 

Qiu X, Tang Y, Yue Y, Zeng Y, Li W, Qu Y et al (2019) Accuracy of interferon-γ-induced protein 10 for diagnosing latent tuberculosis infection: a systematic review and meta-analysis. Clin Microbiol Infect 25:667–672. https://doi.org/10.1016/J.CMI.2018.12.006

Article  CAS  PubMed  Google Scholar 

Chegou NN, Heyckendorf J, Walzl G, Lange C, Ruhwald M (2014) Beyond the IFN-γ horizon: biomarkers for immunodiagnosis of infection with Mycobacterium tuberculosis. Eur Respir J 43:1472–1486. https://doi.org/10.1183/09031936.00151413

Article  CAS  PubMed  Google Scholar 

Ruhwald M, Dominguez J, Latorre I, Losi M, Richeldi L, Pasticci MB et al (2011) A multicentre evaluation of the accuracy and performance of IP-10 for the diagnosis of infection with M. tuberculosis. Tuberculosis 91:260–267. https://doi.org/10.1016/J.TUBE.2011.01.001

Article  PubMed  Google Scholar 

Ruhwald M, Aabye MG, Ravn P (2012) IP-10 release assays in the diagnosis of tuberculosis infection: current status and future directions. Expert Rev Mol Diagn 12:175–187. https://doi.org/10.1586/ERM.11.97

Article  CAS  PubMed  Google Scholar 

Petrone L, Vanini V, Chiacchio T, Petruccioli E, Cuzzi G, Schininà V et al (2018) Evaluation of IP-10 in Quantiferon-Plus as biomarker for the diagnosis of latent tuberculosis infection. Tuberculosis 111:147–153. https://doi.org/10.1016/j.tube.2018.06.005

Article  CAS  PubMed  Google Scholar 

Ortakoylu MG, Bahadir A, Iliaz S, Bugdayci DS, Uysal MA, Paker N et al (2022) Interferon-Inducible Protein-10 as a Marker to Detect Latent Tuberculosis Infection in Patients with Inflammatory Rheumatic Diseases. J Pers Med 12. https://doi.org/10.3390/JPM12071027

Aabye MG, Ruhwald M, PrayGod G, Jeremiah K, Faurholt-Jepsen M, Faurholt-Jepsen D et al (2010) Potential of interferon-γ-inducible protein 10 in improving tuberculosis diagnosis in HIV-infected patients. Eur Respir J 36:1488–1490. https://doi.org/10.1183/09031936.00039010

Article  CAS  PubMed  Google Scholar 

da Silva JCB, Vieira NB, Ribeiro-Alves M, de Almeida Ribeiro RS, da Silva Lemos CC, Mendes R et al Use of interferon-gamma release assay (IGRA) and CXCL-10/IP-10 for latent tuberculosis infection (LTBI) screening in chronic kidney disease and hemodialysis patients. BMC Infect Dis 2025 25:1 2025;25:1224–. https://doi.org/10.1186/S12879-025-11620-Z

Xu F, Ni M, Qu S, Duan Y, Zhang H, Qin Z (2022) Molecular markers of tuberculosis and their clinical relevance: a systematic review and meta-analysis. Ann Palliat Med 11:532–543. https://doi.org/10.21037/APM-21-3739

Article  PubMed  Google Scholar 

Druszczynska M, Seweryn M, Wawrocki S, Pankowska A, Kulbachko A, Jurczak M et al (2025) Interferon (IFN)-gamma (γ) inducible protein 10 (IP-10) in the diagnosis of latent and active tuberculosis in Bacille Calmette Guerin (BCG)-vaccinated pediatric population. PLoS ONE 20:e0314400. https://doi.org/10.1371/JOURNAL.PONE.0314400

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tang Y, Yu Y, Wang Q, Wen Z, Song R, Li Y et al (2023) Evaluation of the IP-10 mRNA release assay for diagnosis of TB in HIV-infected individuals. Front Cell Infect Microbiol 13:1152665. https://doi.org/10.3389/FCIMB.2023.1152665/BIBTEX

Article  CAS  PubMed  PubMed Central  Google Scholar 

Comments (0)

No login
gif