Approaches to visualize, quantify, and manipulate phosphoinositides in cells

A GV, Hwang D, Chakrasali P, Jung E, Lee JY, Shin JS, Jung YS (2024) Highly potent and selective phosphatidylinositol 4-kinase IIIbeta inhibitors as broad-spectrum anti-rhinoviral agents. RSC Med Chem 15(2):704–719. https://doi.org/10.1039/d3md00630a

Article  CAS  Google Scholar 

Algar WR, Hildebrandt N, Vogel SS, Medintz IL (2019) FRET as a biomolecular research tool - understanding its potential while avoiding pitfalls. Nat Methods 16(9):815–829. https://doi.org/10.1038/s41592-019-0530-8

Article  CAS  PubMed  Google Scholar 

Arcaro A, Wymann MP (1993) Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses. Biochem J 296(Pt 2)(Pt 2):297–301. https://doi.org/10.1042/bj2960297

Article  CAS  PubMed  PubMed Central  Google Scholar 

Atak MF, Farabi B, Navarrete-Dechent C, Rubinstein G, Rajadhyaksha M, Jain M (2023) Confocal microscopy for diagnosis and management of cutaneous malignancies: clinical impacts and innovation. Diagnostics Basel. https://doi.org/10.3390/diagnostics13050854

Article  PubMed  PubMed Central  Google Scholar 

Balaban C, Sztacho M, Blazikova M, Hozak P (2021) The F-Actin-binding MPRIP forms phase-separated condensates and associates with PI(4,5)P2 and active RNA polymerase II in the cell nucleus. Cells. https://doi.org/10.3390/cells10040848

Article  PubMed  PubMed Central  Google Scholar 

Balaban C, Sztacho M, Antiga L, Miladinovic A, Harata M, Hozak P (2023) PIP2-effector protein MPRIP regulates RNA polymerase II condensation and transcription. Biomolecules. https://doi.org/10.3390/biom13030426

Article  PubMed  PubMed Central  Google Scholar 

Balla T (2013) Phosphoinositides: tiny lipids with giant impact on cell regulation. Physiol Rev 93(3):1019–1137. https://doi.org/10.1152/physrev.00028.2012

Article  CAS  PubMed  PubMed Central  Google Scholar 

Balla A, Tuymetova G, Tsiomenko A, Varnai P, Balla T (2005) A plasma membrane pool of phosphatidylinositol 4-phosphate is generated by phosphatidylinositol 4-kinase type-III alpha: studies with the PH domains of the oxysterol binding protein and FAPP1. Mol Biol Cell 16(3):1282–1295. https://doi.org/10.1091/mbc.e04-07-0578

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barneda D, Janardan V, Swales J, Ciaccia M, Goodwin R, Cosulich S, Raghu P, Clark J, Stephens L, Hawkins P (2025) Phosphoinositide acyl chain diversity: comparative analysis across species and mouse tissues. Biochim Biophys Acta Mol Cell Biol Lipids 1870(6):159640. https://doi.org/10.1016/j.bbalip.2025.159640

Article  CAS  PubMed  Google Scholar 

Barrangou R, Birmingham A, Wiemann S, Beijersbergen RL, Hornung V, Smith A (2015) Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res 43(7):3407–3419. https://doi.org/10.1093/nar/gkv226

Article  CAS  PubMed  PubMed Central  Google Scholar 

Beresford NJ, Saville C, Bennett HJ, Roberts IS, Tabernero L (2010) A new family of phosphoinositide phosphatases in microorganisms: identification and biochemical analysis. BMC Genomics 11(1):457. https://doi.org/10.1186/1471-2164-11-457

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bojjireddy N, Botyanszki J, Hammond G, Creech D, Peterson R, Kemp DC, Snead M, Brown R, Morrison A, Wilson S, Harrison S, Moore C, Balla T (2014) Pharmacological and genetic targeting of the PI4KA enzyme reveals its important role in maintaining plasma membrane phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate levels. J Biol Chem 289(9):6120–6132. https://doi.org/10.1074/jbc.M113.531426

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bolognesi B, Lehner B (2018) Reaching the limit. Elife. https://doi.org/10.7554/eLife.39804

Article  PubMed  PubMed Central  Google Scholar 

Botero S, Chiaroni-Clarke R, Simon SM (2019) Escherichia coli as a platform for the study of phosphoinositide biology. Sci Adv 5(3):eaat4872. https://doi.org/10.1126/sciadv.aat4872

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bravo J, Karathanassis D, Pacold CM, Pacold ME, Ellson CD, Anderson KE, Butler PJ, Lavenir I, Perisic O, Hawkins PT, Stephens L, Williams RL (2001) The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate. Mol Cell 8(4):829–839. https://doi.org/10.1016/s1097-2765(01)00372-0

Article  CAS  PubMed  Google Scholar 

Broussard JA, Green KJ (2017) Research techniques made simple: methodology and applications of Förster resonance energy transfer (FRET) microscopy. J Invest Dermatol 137(11):e185–e191. https://doi.org/10.1016/j.jid.2017.09.006

Article  CAS  PubMed  PubMed Central  Google Scholar 

Burd CG, Emr SD (1998) Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol Cell 2(1):157–162. https://doi.org/10.1016/s1097-2765(00)80125-2

Article  CAS  PubMed  Google Scholar 

Cai X, Xu Y, Cheung AK, Tomlinson RC, Alcazar-Roman A, Murphy L, Billich A, Zhang B, Feng Y, Klumpp M, Rondeau JM, Fazal AN, Wilson CJ, Myer V, Joberty G, Bouwmeester T, Labow MA, Finan PM, Porter JA, Cheung A K, Tomlinson R C, Fazal A N, Wilson C J, Labow M A, Finan P M, Porter J A, Ploegh H L, Baird D, De Camilli P, Tallarico J A, Huang Q (2013) PIKfyve, a class III PI kinase, is the target of the small molecular IL-12/IL-23 inhibitor apilimod and a player in Toll-like receptor signaling. Chem Biol 20(7):912–921. https://doi.org/10.1016/j.chembiol.2013.05.010

Article  CAS  PubMed  PubMed Central  Google Scholar 

Caires R, Bell B, Lee J, Romero LO, Vasquez V, Cordero-Morales JF (2021) Deficiency of inositol monophosphatase activity decreases phosphoinositide lipids and enhances TRPV1 function in vivo. J Neurosci 41(3):408–423. https://doi.org/10.1523/JNEUROSCI.0803-20.2020

Article  CAS  PubMed  Google Scholar 

Casalin I, Ceneri E, Ratti S, Manzoli L, Cocco L, Follo MY (2024) Nuclear phospholipids and signaling: an update of the story. Cells. https://doi.org/10.3390/cells13080713

Article  PubMed  PubMed Central  Google Scholar 

Castano E, Yildirim S, Faberova V, Krausova A, Ulicna L, Paprckova D, Sztacho M, Hozak P (2019) Nuclear phosphoinositides-versatile regulators of genome functions. Cells 8(7):649. https://doi.org/10.3390/cells8070649

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen Q, Xu Y, Wu JW, Yang JM, Huang CH (2025) Decoding molecular network dynamics in cells: advances in multiplexed live imaging of fluorescent biosensors. Biosensors (Basel) 15(9):614. https://doi.org/10.3390/bios15090614

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chi ES, Stivison EA, Blind RD (2023) SF-1 induces nuclear PIP2. Biomolecules. https://doi.org/10.3390/biom13101509

Article  PubMed  PubMed Central  Google Scholar 

Chytla A, Rattay S, Akgul B, Sztacho M (2025) Plasma membrane and nuclear phosphatidylinositol 4,5-bisphosphate signalling in cancer. Lipids Health Dis 24(1):39. https://doi.org/10.1186/s12944-025-02452-6

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cicchetti G, Biernacki M, Farquharson J, Allen PG (2004) A ratiometric expressible FRET sensor for phosphoinositides displays a signal change in highly dynamic membrane structures in fibroblasts. Biochemistry 43(7):1939–1949. https://doi.org/10.1021/bi035480w

Article  CAS  PubMed  Google Scholar 

Costa C, Ebi H, Martini M, Beausoleil SA, Faber AC, Jakubik CT, Huang A, Wang Y, Nishtala M, Hall B, Rikova K, Zhao J, Hirsch E, Benes CH, Engelman JA (2015) Measurement of PIP3 levels reveals an unexpected role for p110beta in early adaptive responses to p110alpha-specific inhibitors in luminal breast cancer. Cancer Cell 27(1):97–108. https://doi.org/10.1016/j.ccell.2014.11.007

Comments (0)

No login
gif