Abskharon R, Sawaya MR, Boyer DR, Cao Q, Nguyen BA, Cascio D, Eisenberg DS (2022) Cryo-EM structure of RNA-induced tau fibrils reveals a small C-terminal core that may nucleate fibril formation. Proc Natl Acad Sci USA 119:e2119952119

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ambadipudi S, Biernat J, Riedel D, Mandelkow E, Zweckstetter M (2017) Liquid-liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein tau. Nat Commun 8:275

Article  PubMed  PubMed Central  Google Scholar 

Babinchak WM, Haider R, Dumm BK, Sarkar P, Surewicz K, Choi J-K, Surewicz WK (2019) The role of liquid–liquid phase separation in aggregation of the TDP-43 low-complexity domain. J Biol Chem 294:6306–6317

Article  CAS  PubMed  PubMed Central  Google Scholar 

Balmik AA, Das R, Dangi A, Gorantla NV, Marelli UK, Chinnathambi C (2020) Biochim Biophys Acta 1864:129467

Article  CAS  Google Scholar 

Banani SF, Lee HO, Hyman AA, Rosen MK (2017) Biomolecular condensates: organizers of cellular biochemistry. Nat Rev Mol Cell Biol 18:285–298

Article  CAS  PubMed  PubMed Central  Google Scholar 

Basith S, Manavalan B, Lee G (2022) Amyotrophic lateral sclerosis disease-related mutations disrupt the dimerization of superoxide dismutase 1—a comparative molecular dynamics simulation study. Comput Biol Med 151:106319

Article  CAS  PubMed  Google Scholar 

Benetti R, Gonzalo S, Jaco I, Schotta G, Klatt P, Jenuwein T, Blasco MA (2007) Suv4-20h deficiency results in telomere elongation and derepression of telomere recombination. J Cell Biol 178:925–936

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bentley EP, Frey BB, Deniz AA (2019) Physical chemistry of cellular liquid phase separation. Chemistry 25:5600–5610

Article  CAS  PubMed  PubMed Central  Google Scholar 

Box JK, Paque TN, Adams MN, Boucher D, Bolderson E, O’Byrne KJ, Richard DJ (2016) Nucleophosmin: from structure and function to disease development. BMC Mol Biol 24(17(1)):19

Article  Google Scholar 

Boyko S, Qi X, Chen T-H, Surewicz K, Surewicz WK (2019) Liquid–liquid phase separation of tau protein: the crucial role of electrostatic interactions. J Biol Chem 294:11054–11059

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brocca S, Grandori R, Longhi S, Uversky V (2020) Liquid–liquid phase separation by intrinsically disordered protein regions of viruses: roles in viral life cycle and control of virus–host interactions. Int J Mol Sci 21:9045

Article  CAS  PubMed  PubMed Central  Google Scholar 

Burley SK, Petsko GA (1988) Weakly polar interactions in proteins. Adv Prot Chem 39:125–189

CAS  Google Scholar 

Capper MJ, Wright GSA, Barbieri L, Luchinat E, Mercatelli E, McAlary L, Yerbury JJ, O’Neill PM, Antonyuk SV, Banci L, Hasnain SS (2018) The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation. Nat Commun 9:1693

Article  PubMed  PubMed Central  Google Scholar 

Cario A, Berger CL (2023) Tau, microtubule dynamics, and axonal transport: new paradigms for neurodegenerative disease. BioEssays 45:2200138

Article  CAS  Google Scholar 

Charafeddine RA, Cortopassi WA, Lak P, Tan R, McKenney RJ, Jacobson MP, Barber DL, Wittmann T (2019) Tau repeat regions contain conserved histidine residues that modulate microtubule-binding in response to changes in pH. J Biol Chem 294:8779–8790

Article  PubMed  PubMed Central  Google Scholar 

Chen C, Yamanaka Y, Ueda K, Li P, Miyagi T, Harada Y, Tezuka S, Narumi S, Sugimoto M, Kuroda M, Hayamizu Y, Kanekura K (2021) Phase separation and toxicity of C9orf72 poly (PR) depends on alternate distribution of arginine. J Cell Biol 220:e202103160

Article  CAS  PubMed  PubMed Central  Google Scholar 

Conicella AE, Zerze GH, Mittal J, Fawzi NL (2016) ALS mutations disrupt phase separation mediated by α-helical structure in the TDP-43 low-complexity C-terminal domain. Structure 24:1537–1549

Article  CAS  PubMed  PubMed Central  Google Scholar 

Conicella AE, Dignon GL, Zerze GH, Schmidt HB, D’Ordine AM, Kim YC, Rohatgi R, Ayala YM, Mittal J, Fawzi NL (2020) TDP-43 α-helical structure tunes liquid–liquid phase separation and function. Proc Natl Acad Sci USA 117:5883–5894

Article  CAS  PubMed  PubMed Central  Google Scholar 

Crabtree MD, Holland J, Kompella P, Babl L, Turner N, Baldwin AJ, Nott TJ (2021) Repulsive electrostatic interactions modulate dense and dilute phase properties of biomolecular condensates. bioRxiv preprint. https://doi.org/10.1101/2020.10.29.357863

Danielsson J, Kurnik M, Lang L, Oliveberg M (2011) Cutting off functional loops from Homodimeric enzyme superoxide dismutase 1 (SOD1) leaves monomeric-barrels. J Biol Chem 286:33070–33083

Article  CAS  PubMed  PubMed Central  Google Scholar 

Das S, Lin Y-H, Vernon RM, Forman-Kay JD, Chan HS (2020) Comparative roles of charge, π, and hydrophobic interactions in sequence-dependent phase separation of intrinsically disordered proteins. Proc Natl Acad Sci USA 117:28795–28805

Article  CAS  PubMed  PubMed Central  Google Scholar 

Das B, Roychowdhury S, Mohanty P, Rizuan A, Chakraborty J, Mittal J, Chattopadhyay K (2022) Zn-dependent structural transition of SOD1 modulates its ability to undergo liquid-liquid phase separation. EMBO J 42:e111185

Article  PubMed  PubMed Central  Google Scholar 

Dinkel PD, Holden MR, Matin N, Margittai M (2015) RNA binds to tau fibrils and sustains template-assisted growth. Biochemistry 54:4731–4740

Article  CAS  PubMed  Google Scholar 

El Mammeri N, Dregni AJ, Duan P, Wang HK, Hong M (2022) Microtubule-binding core of the tau protein. Sci Adv 8:eabo4459

Article  PubMed  PubMed Central  Google Scholar 

Feric M, Vaidya N, Harmon TS, Mitrea DM, Zhu L, Richardson TM, Kriwacki RW, Pappu RV, Brangwynne CP (2016) Coexisting liquid phases underlie nucleolar sub compartments. Cell 165:1686–1697

Article  CAS  PubMed  PubMed Central  Google Scholar 

Getzoff ED, Tainer JA, Weiner PK, Kollman PA, Richardson JS, Richardson DC (1983) Electrostatic recognition between superoxide and copper, zinc superoxide dismutase. Nature 306:287–290

Article  CAS  PubMed  Google Scholar 

Getzoff ED, Cabelli DE, Fisher CL, Parge HE, Viezzoli MS, Banci L, Hallewell RA (1992) Faster superoxide dismutase mutants designed by enhancing electrostatic guidance. Nature 358:347–351

Article  CAS  PubMed  Google Scholar 

Gobbi A, Frenking G (1993) Y-conjugated compounds: the equilibrium geometries and electronic structures of guanidine, guanidinium cation, urea, and 1,1-diaminoethylene. J Am Chem Soc 115:2362–2372

Article  CAS  Google Scholar 

Gomes E, Shorter J (2019) The molecular language of membraneless organelles. J Biol Chem 294:7115–7127

Article  CAS  PubMed  Google Scholar 

Gomez-Tortosa E, Gallego J, Guerrero-Lopez R, Marcos A, Gil-Neciga E, Sainz MJ, Diaz A, Franco-Macias E, Trujillo-Tiebas MJ, Ayuso C, Perez-Perez J (2013) C9ORF72 hexanucleotide expansions of 20–22 repeats are associated with frontotemporal deterioration. Neurology 80:366–370

Article  CAS  PubMed  Google Scholar 

Greig JA, Nguyen TA, Lee M, Holehouse A, Posey AE, Pappu RV, Jedd G (2020) Arginine-enriched mixed-charge domains provide cohesion for nuclear speckle condensation. Mol Cell 77:1237–1250

Article  CAS