Tight junction structure, assembly and (dys)function

Anderson, J. M. & Van Itallie, C. M. Physiology and function of the tight junction. Cold Spring Harb. Perspect. Biol. 1, a002584 (2009).

Article  PubMed  PubMed Central  Google Scholar 

Citi, S. et al. A short guide to the tight junction. J. Cell Sci. 137, jcs261776 (2024).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Günzel, D. & Yu, A. S. L. Claudins and the modulation of tight junction permeability. Physiol. Rev. 93, 525–569 (2013).

Article  PubMed  PubMed Central  Google Scholar 

Fanning, A. S. & Anderson, J. M. Zonula occludens-1 and -2 are cytosolic scaffolds that regulate the assembly of cellular junctions. Ann. N. Y. Acad. Sci. 1165, 113–120 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Maiers, J. L., Peng, X., Fanning, A. S. & DeMali, K. A. ZO-1 recruitment to α-catenin — a novel mechanism for coupling the assembly of tight junctions to adherens junctions. J. Cell Sci. 126, 3904–3915 (2013).

CAS  PubMed  PubMed Central  Google Scholar 

Ooshio, T. et al. Involvement of the interaction of afadin with ZO-1 in the formation of tight junctions in Madin–Darby canine kidney cells. J. Biol. Chem. 285, 5003–5012 (2010).

Article  CAS  PubMed  Google Scholar 

Gumbiner, B. & Simons, K. A functional assay for proteins involved in establishing an epithelial occluding barrier: identification of a uvomorulin-like polypeptide. J. Cell Biol. 102, 457–468 (1986).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Suzuki, A. et al. Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures. J. Cell Biol. 152, 1183–1196 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fogg, V. C., Liu, C.-J. & Margolis, B. Multiple regions of Crumbs3 are required for tight junction formation in MCF10A cells. J. Cell Sci. 118, 2859–2869 (2005).

Article  CAS  PubMed  Google Scholar 

Rojas, R., Ruiz, W. G., Leung, S.-M., Jou, T.-S. & Apodaca, G. Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin–Darby canine kidney cells. Mol. Biol. Cell 12, 2257–2274 (2001).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pombo-García, K., Adame-Arana, O., Martin-Lemaitre, C., Jülicher, F. & Honigmann, A. Membrane prewetting by condensates promotes tight-junction belt formation. Nature 632, 647–655 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Nguyen, T. P. et al. Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complex. J. Cell Biol. 223, e202307104 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Balda, M. S. & Matter, K. Tight junctions at a glance. J. Cell Sci. 121, 3677–3682 (2008).

Article  CAS  PubMed  Google Scholar 

Stephenson, R. E. et al. Rho flares repair local tight junction leaks. Dev. Cell 48, 445–459.e5 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Varadarajan, S. et al. Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling. J. Cell Biol. 221, e202105107 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gonschior, H. et al. Nanoscale segregation of channel and barrier claudins enables paracellular ion flux. Nat. Commun. 13, 4985 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Beutel, O., Maraspini, R., Pombo-García, K., Martin-Lemaitre, C. & Honigmann, A. Phase separation of zonula occludens proteins drives formation of tight junctions. Cell 179, 923–936.e11 (2019).

Article  CAS  PubMed  Google Scholar 

Schwayer, C. et al. Mechanosensation of tight junctions depends on ZO-1 phase separation and flow. Cell 179, 937–952.e18 (2019).

Article  CAS  PubMed  Google Scholar 

Sun, D. et al. Assembly of tight junction belts by ZO1 surface condensation and local actin polymerization. Dev. Cell 60, 1234–1250.e6 (2025).

Article  CAS  PubMed  Google Scholar 

Suzuki, H., Tani, K., Tamura, A., Tsukita, S. & Fujiyoshi, Y. Model for the architecture of claudin-based paracellular ion channels through tight junctions. J. Mol. Biol. 427, 291–297 (2015).

Article  CAS  PubMed  Google Scholar 

Suzuki, H. et al. Crystal structure of a claudin provides insight into the architecture of tight junctions. Science 344, 304–307 (2014).

Article  CAS  PubMed  Google Scholar 

Otani, T. et al. Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity. J. Cell Biol. 218, 3372–3396 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Spadaro, D. et al. Tension-dependent stretching activates ZO-1 to control the junctional localization of its interactors. Curr. Biol. 27, 3783–3795.e8 (2017).

Article  CAS  PubMed  Google Scholar 

Mangeol, P. et al. Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia. eLife 11, e62087 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mangeol, P. et al. The zonula adherens matura redefines the apical junction of intestinal epithelia. Proc. Natl Acad. Sci. USA 121, e2316722121 (2024).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tan, B. et al. The mammalian Crumbs complex defines a distinct polarity domain apical of epithelial tight junctions. Curr. Biol. 30, 2791–2804.e6 (2020).

Article  CAS  PubMed  Google Scholar 

Utepbergenov, D. I., Fanning, A. S. & Anderson, J. M. Dimerization of the scaffolding protein ZO-1 through the second PDZ domain. J. Biol. Chem. 281, 24671–24677 (2006).

Article  CAS  PubMed  Google Scholar 

Fanning, A. S., Lye, M. F., Anderson, J. M. & Lavie, A. Domain swapping within PDZ2 is responsible for dimerization of ZO proteins. J. Biol. Chem. 282, 37710–37716 (2007).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Umeda, K. et al. ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell 126, 741–754 (2006).

Article  CAS  PubMed  Google Scholar 

Fanning, A. S., Ma, T. Y. & Anderson, J. M. Isolation and functional characterization of the actin binding region in the tight junction protein ZO-1. FASEB J. 16, 1835–1837 (2002).

Article  CAS  PubMed 

Comments (0)

No login
gif