Al-Khayri JM, Narasimha SW, Vennapusa AR, Nagella P, Shehata WF, Al-massallem MQ (2024) Biotechnological approaches for the production of hypericin and other important metabolites from the genus Hypericum. Plant Cell Tiss Org Cult 156:1–18. https://doi.org/10.1007/s11240-024-02723-7

Article  CAS  Google Scholar 

Amooaghaie R, Rajaie N (2025) Exploring effect of chitosan on antioxidant system and hypericin content in Hypericum perforatum L. under various irrigation regimes. BMC Plant Biol 25:799. https://doi.org/10.1186/s12870-025-06887-y

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bais HP, Vepachedu R, Lawrence CB, Stermitz FR, Vivanco JM (2003) Molecular and biochemical characterization of an enzyme responsible for the formation of hypericin in St. John’s wort (Hypericum perforatum L.). J Biol Chem 278:32413–32422. https://doi.org/10.1074/jbc.M301681200

Article  CAS  PubMed  Google Scholar 

Barnes J, Anderson LA, Phillipson JD (2001) St John’s wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J Pharm Pharmacol 53:583–600. https://doi.org/10.1211/0022357011775910

Article  CAS  PubMed  Google Scholar 

Biswas D, Chakraborty A, Mukherjee S, Ghosh B (2023) Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants. Front Plant Sci 14:1197555. https://doi.org/10.3389/fpls.2023.1197555

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brasili E, Miccheli A, Marini F, Praticò G, Sciubba F, Di Cocco ME, Cechinel VF, Tocci N, Valletta A, Pasqua G (2016) Metabolic profile and root development of Hypericum perforatum L. in vitro roots under stress conditions due to chitosan treatment and culture time. Front Plant Sci 7:507. https://doi.org/10.3389/fpls.2016.00507

Article  PubMed  PubMed Central  Google Scholar 

Butterweck V (2003) Mechanism of action of St John’s wort in depression. CNS Drugs 17:539–562. https://doi.org/10.2165/00023210-200317080-00001

Article  CAS  PubMed  Google Scholar 

Chayjarung P, Phonherm M, Inmano O, Kongbangkerd A, Wongsa T, Limmongkon A (2022) Influence of peanut hairy root cultivars on prenylated stilbenoid production and the response mechanism for combining the elicitors of chitosan, methyl jasmonate, and cyclodextrin. Planta 256:32. https://doi.org/10.1007/s00425-022-03946-8

Article  CAS  PubMed  Google Scholar 

Coelho N, Romano A (2022) Impact of chitosan on plant tissue culture: recent applications. Plant Cell Tiss Org Cult 148:1–13. https://doi.org/10.1007/s11240-021-02156-6

Article  CAS  Google Scholar 

Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010b) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol 101:4708–4716. https://doi.org/10.1016/j.biortech.2010.01.115

Article  CAS  PubMed  Google Scholar 

Cui XH, Murthy HN, Wu CH, Paek KY (2010a) Adventitious root suspension cultures of Hypericum perforatum: effect of nitrogen source on production of biomass and secondary metabolites. In Vitro Cell Dev Biol - Plant 46:437–444. https://doi.org/10.1007/s11627-010-9310-y

Article  CAS  Google Scholar 

Gadzovska S, Maury S, Delaunay A, Spasenoski M, Joseph C, Hagège D (2007) Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tiss Org Cult 89:1–13. https://doi.org/10.1007/s11240-007-9203-x

Article  CAS  Google Scholar 

Gadzovska Simic S, Tusevski O, Maury S, Delaunay A, Joseph C, Hagège D (2015) Polysaccharide elicitors enhance phenylpropanoid and naphtodianthrone production in cell suspension cultures of Hypericum perforatum. Plant Cell Tiss Org Cult 122:649–663. https://doi.org/10.1007/s11240-015-0798-z

Article  CAS  Google Scholar 

Georgiev MI, Ludwig-Müller J, Bley T (2010) Hairy root culture: copying nature in new bioprocesses. In Medicinal plant biotechnology CABI Wallingford, United Kingdom.

Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1–22. https://doi.org/10.1016/s0734-9750(99)00016-6

Article  CAS  PubMed  Google Scholar 

Gjureci B, Todorovska M, Stanoeva JP, Stefova M, Tusevski O, Simic SG (2025) Elicitation of Hypericum perforatum L. hairy root cultures with salicylic acid and jasmonic acid enhances the production of phenolic compounds and naphthodianthrones with biological activities. Plant Cell Tiss Org Cult 160:1–27. https://doi.org/10.1007/s11240-025-03005-6

Article  CAS  Google Scholar 

Guillon S, Trémouillaux-Guiller J, Pati PK, Rideau M, Gantet P (2006) Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol 9:341–346. https://doi.org/10.1016/j.pbi.2006.03.008

Article  CAS  PubMed  Google Scholar 

Gutierrez-Valdes N, Häkkinen ST, Lemasson C, Guillet M, Oksman-Caldentey KM, Ritala A, Cardon F (2020) Hairy root cultures—a versatile tool with multiple applications. Front Plant Sci 11:33. https://doi.org/10.3389/fpls.2020.00033

Article  PubMed  PubMed Central  Google Scholar 

He M, Wang Y, Hua W, Zhang Y, Wang Z (2012) De novo sequencing of Hypericum perforatum transcriptome to identify potential genes involved in the biosynthesis of active metabolites. PLoS ONE 7:e42081. https://doi.org/10.1371/journal.pone.0042081

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hussain MJ, Abbas Y, Nazli N, Fatima S, Drouet S, Hano C, Abbasi BH (2022) Root cultures, a boon for the production of valuable compounds: a comparative review. Plants 11:439. https://doi.org/10.3390/plants11030439

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jiao J, Gai QY, Wang X, Qin QP, Wang ZY, Liu J, Fu YJ (2018) Chitosan elicitation of Isatis tinctoria L. hairy root cultures for enhancing flavonoid productivity and gene expression and related antioxidant activity. Ind Crops Prod 124:28–35. https://doi.org/10.1016/j.indcrop.2018.07.056

Article  CAS  PubMed  PubMed Central  Google Scholar 

Karppinen K, Hohtola A (2008) Molecular cloning and tissue-specific expression of two cDNAs encoding polyketide synthases from Hypericum perforatum. J Plant Physiol 165:1079–1086. https://doi.org/10.1016/j.jplph.2007.04.008

Article  CAS  PubMed  Google Scholar 

Kirakosyan A, Sirvent TM, Gibson DM, Kaufman PB (2004) The production of hypericins and hyperforin by in vitro cultures of St. John’s wort (Hypericum perforatum). Biotechnol Appl Biochem 39:71–81. https://doi.org/10.1042/BA20030144

Article  CAS  PubMed  Google Scholar 

Košuth J, Hrehorová D, Jaskolski M, Cellárová E (2013) Stress-induced expression and structure of the putative gene Hyp1 for hypericin biosynthesis. Plant Cell Tiss Org Cult 114:207–216. https://doi.org/10.1007/s11240-013-0316-0

Article  CAS  Google Scholar 

Kosuth J, Katkovcinová Z, Olexová P, Cellárová E (2007) Expression of the Hyp1 gene in early stages of development of Hypericum perforatum L. Plant Cell Rep 26:211–217. https://doi.org/10.1007/s00299-006-0240-4

Article  CAS  PubMed  Google Scholar 

Loganathan K, Seethapathy P, Pandita D, Packiaraj S, Venkatesh S, Sankaralingam S, Arunkumar M, Harinathan B, Pandita A, Casini R, Mahmoud EA, Moussa IM, Elansary HO (2025) Chitosan (CTS) induced secondary metabolite production in Canscora decussata Schult. an endangered medicinal plant. Kuwait J Sci 52:100306. https://doi.org/10.1016/j.kjs.2024.100306

Article  Google Scholar 

Montazeri M, Azadbakht M, Najafi Zarrini H, NematZadeh GA, Pakdin Parizi A, Davoodi A (2018) Hairy root cultures of Hypericum perforatum l.; a promising method for the high scale production of hypericin. J Med Plants 17:55–67

Google Scholar 

Murthy HN, Kim YS, Park SY, Paek KY (2014) Hypericins: biotechnological production from cell and organ cultures. Appl Microbiol Biotechnol 98:9187–9198. https://doi.org/10.1007/s00253-014-6119-3

Article  CAS  PubMed  Google Scholar 

Nigutová K, Kusari S, Sezgin S, Petijová L, Henzelyová J, Bálintová M, Spiteller M, Čellárová E (2019) Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants. J Pharm Pharmacol 71:46–57. https://doi.org/10.1111/jphp.12782

Article  CAS  PubMed