Salerno M, Cocimano G, Roccuzzo S et al (2022) New trends in immunohistochemical methods to estimate the time since death: a review. Diagnostics 12. https://doi.org/10.3390/diagnostics12092114

Guebelin DLC, Dobay A, Ebert L et al (2021) Correlation of age, sex and season with the state of human decomposition as quantified by postmortem computed tomography. Forensic Sci Med Pathol 17:185–191. https://doi.org/10.1007/s12024-021-00356-2

Article  PubMed  PubMed Central  Google Scholar 

Marrone A, La Russa D, Barberio L et al (2023) Forensic proteomics for the discovery of new post mortem interval biomarkers: a preliminary study. Int J Mol Sci. https://doi.org/10.3390/ijms241914627

Article  PubMed  PubMed Central  Google Scholar 

Shrestha R, Kanchan T, Krishan K (2023) Methods of estimation of time since death

Chheda U, Pradeepan S, Esposito E et al (2024) Factors affecting stability of RNA – temperature, length, concentration, pH, and buffering species. J Pharm Sci 113:377–385. https://doi.org/10.1016/j.xphs.2023.11.023

Article  PubMed  Google Scholar 

Yasojima K, Mcgeer EG, Mcgeer PL (2001) High stability of mRNAs postmortem and protocols for their assessment by RT-PCR. https://doi.org/10.1016/s1385-299x(01)00119-2

Elghamry HA, Mohamed MI, Hassan FM et al (2017) Potential use of GAPDH m-RNA in estimating PMI in brain tissue of albino rats at different environmental conditions. Egypt J Forensic Sci. https://doi.org/10.1186/s41935-017-0024-8

Article  Google Scholar 

Ebrahimi R, Bahiraee A, Emamgholipour S et al Evaluation of the Housekeeping Genes; β-Actin, Glyceraldehyde-3-Phosphate-Dehydrogenase, and 18S rRNA for Normalization in Real-Time Polymerase Chain Reaction Analysis of Gene Exp… Evaluation of the Housekeeping Genes; β-Actin, Glyceraldehyde-3-Phosphate-Dehydrogenase, and 18S rRNA for Normalization in Real-Time Polymerase Chain Reaction Analysis of Gene Expression in Human Adipose Tissue. https://doi.org/10.22037/amls.v4i3.26269

Liu Y, Beyer A, Aebersold R (2016) On the dependency of cellular protein levels on mRNA abundance. Cell 165:535–550

Article  PubMed  Google Scholar 

Pasaribu RS, Auerkari EI, Suhartono AW, Auerkari P (2023) A small RNA, MicroRNA as a potential biomolecular marker to estimate post mortem interval in forensic science: a systematic review. Int J Legal Med 137:1313–1325

Article  PubMed  PubMed Central  Google Scholar 

Kornienko IV, Aramova OYu, Tishchenko AA et al (2024) RNA stability: a review of the role of structural features and environmental conditions. Molecules 29:5978. https://doi.org/10.3390/molecules29245978

Article  PubMed  PubMed Central  Google Scholar 

Matsuyama H, Suzuki HI (2020) Systems and synthetic MicroRNA biology: from biogenesis to disease pathogenesis. Int J Mol Sci 21. https://doi.org/10.3390/ijms21010132

Ying SY, Chang DC, Lin SL (2008) The MicroRNA (miRNA): overview of the RNA genes that modulate gene function. Mol Biotechnol 38:257–268

Article  PubMed  Google Scholar 

Chen X, Xu H, Lin Y, Zhu B (2024) Forensic stability evaluation of selected miRNA and circRNA markers in human bloodstained samples exposed to different environmental conditions. Forensic Sci Int. https://doi.org/10.1016/j.forsciint.2024.112148

Article  PubMed  Google Scholar 

Sampaio-Silva F, Magalhães T, Carvalho F et al (2013) Profiling of RNA degradation for estimation of post morterm interval. PLoS One. https://doi.org/10.1371/journal.pone.0056507

Article  PubMed  PubMed Central  Google Scholar 

Ma J, Pan H, Zeng Y et al (2015) Exploration of the R code-based mathematical model for PMI estimation using profiling of RNA degradation in rat brain tissue at different temperatures. Forensic Sci Med Pathol 11:530–537. https://doi.org/10.1007/s12024-015-9703-7

Article  PubMed  Google Scholar 

Thakral S, Setia P, Modi A et al (2022) Serial estimation of gene expression of cardiac troponin I (cTnI) and autophagy gene HMGB1 to determine postmortem interval. Forensic Sci Int Genet. https://doi.org/10.1016/j.fsigen.2021.102635

Article  PubMed  Google Scholar 

Thakral S, Purohit P, Mishra R et al (2023) The impact of RNA stability and degradation in different tissues to the determination of post-mortem interval: a systematic review. Forensic Sci Int. https://doi.org/10.1016/j.forsciint.2023.111772

Article  PubMed  Google Scholar 

Harshitha R, Arunraj DR (2021) Real-time quantitative PCR: a tool for absolute and relative quantification. Biochem Mol Biol Educ 49:800–812. https://doi.org/10.1002/bmb.21552

Article  PubMed  Google Scholar 

Walker DG, Whetzel AM, Serrano G et al (2016) Characterization of RNA isolated from eighteen different human tissues: results from a rapid human autopsy program. Cell Tissue Bank 17:361–375. https://doi.org/10.1007/s10561-016-9555-8

Article  PubMed  PubMed Central  Google Scholar 

Kim JY, Kim Y, Cha HK et al (2017) Cell death-associated ribosomal RNA cleavage in postmortem tissues and its forensic applications. Mol Cells 40:410–417. https://doi.org/10.14348/molcells.2017.0039

Article  PubMed  PubMed Central  Google Scholar 

Peng D, Lv M, Li Z et al (2020) Postmortem interval determination using mRNA markers and DNA normalization. Int J Legal Med 134:149–157. https://doi.org/10.1007/s00414-019-02199-7

Article  PubMed  Google Scholar 

de Santis C, Smith-Keune C, Jerry DR (2011) Normalizing RT-qPCR data: are we getting the right answers? An appraisal of normalization approaches and internal reference genes from a case study in the finfish Lates calcarifer. Mar Biotechnol 13:170–180. https://doi.org/10.1007/s10126-010-9277-z

Article  Google Scholar 

Xie F, Xiao P, Chen D et al (2012) miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol Biol 80:75–84. https://doi.org/10.1007/s11103-012-9885-2

Article  Google Scholar 

Denti MA, Viero G, Provenzani A et al (2013) mRNA fate: life and death of the mRNA in the cytoplasm. RNA Biol 10:360–366. https://doi.org/10.4161/rna.23770

Article  PubMed  PubMed Central  Google Scholar 

Sulthana S, Basturea GN, Deutscher MP (2016) Elucidation of pathways of ribosomal RNA degradation: an essential role for RNase E. RNA 22:1163–1171. https://doi.org/10.1261/rna.056275.116

Article  PubMed  PubMed Central  Google Scholar 

Pavanello L, Hall M, Winkler GS (2023) Regulation of eukaryotic mRNA deadenylation and degradation by the Ccr4-Not complex. Front Cell Dev Biol 11. https://doi.org/10.3389/fcell.2023.1153624

Catalanotto C, Barbato C, Cogoni C, Benelli D (2023) The RNA-binding function of ribosomal proteins and ribosome biogenesis factors in human health and disease. Biomedicines. https://doi.org/10.3390/biomedicines11112969

Article  PubMed  PubMed Central  Google Scholar 

González-Herrera L, Valenzuela A, Marchal JA et al (2013) Studies on RNA integrity and gene expression in human myocardial tissue, pericardial fluid and blood, and its postmortem stability. Forensic Sci Int 232:218–228. https://doi.org/10.1016/j.forsciint.2013.08.001

Article  PubMed  Google Scholar 

Koppelkamm A, Vennemann B, Fracasso T et al (2010) Validation of adequate endogenous reference genes for the normalisation of qPCR gene expression data in human post mortem tissue. Int J Legal Med 124:371–380. https://doi.org/10.1007/s00414-010-0433-9

Article  PubMed  Google Scholar 

Tozzo P, Scrivano S, Sanavio M, Caenazzo L (2020) The role of DNA degradation in the estimation of post-mortem interval: a systematic review of the current literature. Int J Mol Sci. https://doi.org/10.3390/ijms21103540

Article  PubMed  PubMed Central  Google Scholar 

O'Brien J, Hayder H, Zayed Y, Peng C (2018) Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Front Endocrinol (Lausanne) 9:402. https://doi.org/10.3389/fendo.2018.00402

Wang H, Mao J, Li Y et al (2013) 5 miRNA expression analyze in post-mortem interval (PMI) within 48h. Forensic Science International: Genetics Supplement Series. https://doi.org/10.1016/j.fsigss.2013.10.098

Article  Google Scholar 

Lv YH, Ma KJ, Zhang H et al (2014) A time course study demonstrating mRNA, microRNA, 18S rRNA, and U6 snRNA changes to estimate PMI in deceased rat’s spleen. J Forensic Sci 59:1286–1294. https://doi.org/10.1111/1556-4029.12447

Article  PubMed  Google Scholar 

Guardado-Estrada M, Cárdenas-Monroy CA, Martínez-Rivera V et al (2023) A miRNome analysis at the early postmortem interval. PeerJ. https://doi.org/10.7717/peerj.15409

Article  PubMed  PubMed Central  Google Scholar 

Li WC, Ma KJ, Lv YH et al (2014) Postmortem interval determination using 18S-rRNA and microRNA. Sci Justice 54:307–310. https://doi.org/10.1016/j.scijus.2014.03.001

Article  PubMed  Google Scholar 

Kai ZS, Pasquinelli AE (2010) MicroRNA assassins: factors that regulate the disappearance of miRNAs