Revealing the Immunomodulatory Targets and Mechanisms of Sijing Pill for Postmenopausal Osteoporosis: A UHPLC-MS-Based Bioinformatics and Machine Learning Study

Thumbigere-Math V, Somerman M. Postmenopausal Osteoporosis. N Engl J Med. 2024;390(7):674–5.

PubMed  Google Scholar 

Fang H, Zhang H, Wang Z, et al. Systemic immune-inflammation index acts as a novel diagnostic biomarker for postmenopausal osteoporosis and could predict the risk of osteoporotic fracture. J Clin Lab Anal. 2020;34(1):e23016.

Article  PubMed  Google Scholar 

Jin X, Wang Y, Wang H, et al. Correlation study: Bone density and circulating inflammatory markers in postmenopausal patients. Immun Inflammation Dis. 2024;12(8):e1365.

Article  CAS  Google Scholar 

Gao P, Pan X, Wang S, et al. Identification of the transcriptome signatures and immune-inflammatory responses in postmenopausal osteoporosis. Heliyon. 2024;10(1):e23675.

Article  CAS  PubMed  Google Scholar 

Wang X, Zhang X, Han Y, et al. Role of the major histocompatibility complex class II protein presentation pathway in bone immunity imbalance in postmenopausal osteoporosis. Front Endocrinol (Lausanne). 2022;13:876067.

Article  PubMed  PubMed Central  Google Scholar 

Wang X, Sun B, Wang Y, et al. Research progress of targeted therapy regulating Th17/Treg balance in bone immune diseases. Front Immunol. 2024;15:1333993.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang Y-W, Cao M-M, Li Y-J, et al. Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function. J Orthop Translat. 2022;37:46–60.

Article  PubMed  PubMed Central  Google Scholar 

Brown JP. Long-term treatment of postmenopausal osteoporosis. Endocrinol Metab. 2021;36(3):544–52.

Article  CAS  Google Scholar 

Cosman F. Long-term treatment strategies for postmenopausal osteoporosis. Curr Opin Rheumatol. 2018;30(4):420–6.

Article  CAS  PubMed  Google Scholar 

Tai T-W, Chen H-Y, Shih C-A, et al. Asia-Pacific consensus on long-term and sequential therapy for osteoporosis. Osteoporos Sarcopenia. 2024;10(1):3–10.

Article  PubMed  PubMed Central  Google Scholar 

Anastasilakis AD, Polyzos SA, Yavropoulou MP, et al. Combination and sequential treatment in women with postmenopausal osteoporosis. Expert Opin Pharmacother. 2020;21(4):477–90.

Article  CAS  PubMed  Google Scholar 

Bandeira L, Lewiecki EM. Anabolic therapy for osteoporosis: update on efficacy and safety. Arch Endocrinol Metab. 2022;66(5):707–16.

Article  PubMed  PubMed Central  Google Scholar 

Cosman F. The evolving role of anabolic therapy in the treatment of osteoporosis. Curr Opin Rheumatol. 2019;31(4):376–80.

Article  PubMed  Google Scholar 

Li K, Yu H, Lin X, et al. The effects of Er Xian Decoction combined with Baduanjin Exercise on bone mineral density, lower limb balance function, and mental health in women with postmenopausal osteoporosis: a randomized controlled trial. Evid Based Complement Alternat Med. 2022;2022:1–13.

Google Scholar 

Xc C, Wj Li, Jy Z, et al. Shengu granules ameliorate ovariectomy-induced osteoporosis by the gut-bone-immune axis. Front Microbiol. 2024;15:1320500.

Article  Google Scholar 

Ma Y, Hu J, Song C, et al. Er-Xian decoction attenuates ovariectomy-induced osteoporosis by modulating fatty acid metabolism and IGF1/PI3K/AKT signaling pathway. J Ethnopharmacol. 2023;301:115835-.

Xu H, Tao L, Cao J, et al. Yi Shen Juan Bi Pill alleviates bone destruction in inflammatory arthritis under postmenopausal conditions by regulating ephrinB2 signaling. Front Pharmacol. 2022;13:1010640.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wu F, Li H, Jin L, et al. Deer antler base as a traditional Chinese medicine: a review of its traditional uses, chemistry and pharmacology. J Ethnopharmacol. 2013;145(2):403–15.

Article  PubMed  Google Scholar 

Zhang L-Z, Xin J-L, Zhang X-P, et al. The anti-osteoporotic effect of velvet antler polypeptides from Cervus elaphus Linnaeus in ovariectomized rats. J Ethnopharmacol. 2013;150(1):181–6.

Article  CAS  PubMed  Google Scholar 

Fu Z, Fan X, Wang X, et al. Cistanches Herba: An overview of its chemistry, pharmacology, and pharmacokinetics property. J Ethnopharmacol. 2018;219:233–47.

Article  CAS  PubMed  Google Scholar 

Li J, Zou Z, Su X, et al. Cistanche deserticola improves ovariectomized-induced osteoporosis mainly by regulating lipid metabolism: insights from serum metabolomics using UPLC/Q-TOF-MS. J Ethnopharmacol. 2024;322:117570.

Article  CAS  PubMed  Google Scholar 

Liang H-D, Yu F, Tong Z-H, et al. Cistanches Herba aqueous extract affecting serum BGP and TRAP and bone marrow Smad1 mRNA, Smad5 mRNA, TGF-β1 mRNA and TIEG1 mRNA expression levels in osteoporosis disease. Mol Biol Rep. 2013;40(2):757–63.

Article  CAS  PubMed  Google Scholar 

Liang B, Chen X, Li M, et al. Liuwei Dihuang pills attenuate ovariectomy-induced bone loss by alleviating bone marrow mesenchymal stem cell (BMSC) senescence via the Yes-associated protein (YAP)-autophagy axis. Pharm Biol. 2024;62(1):42–52.

Article  CAS  PubMed  Google Scholar 

Su X, Du X, Guo J, et al. Jianpi gushen fa tanjiu Sijing wan dui juejing hou guzhi shusong dashu de zhiliao zuoyong [A Study on the Therapeutic Effects of Sijing Pill on Postmenopausal Osteoporosis Rats Based on the Method of Tonifying the spleen and kidney]. Chin J Gerontol. 2024;44(09):2193–7 ((in Chinese)).

CAS  Google Scholar 

Jiang T, Li C, Li Y, et al. Multi-omics and bioinformatics for the investigation of therapeutic mechanism of roucongrong pill against postmenopausal osteoporosis. J Ethnopharmacol. 2025;337(Pt 2):118873.

Article  CAS  PubMed  Google Scholar 

Pollastri MP. Overview on the rule of five. Curr Protoc Pharmacol. 2010;Chapter 9:Unit 9.12.1-Unit 9.12.8.

Langfelder P, Horvath S. WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics. 2008;9:559.

Article  PubMed  PubMed Central  Google Scholar 

Zhang B, Horvath S. A general framework for weighted gene co-expression network analysis. Stat Appl Genet Mol Biol. 2005;4:Article17.

Doriguello JF, Lim D, Pun CS, et al. Quantum algorithms for the pathwise Lasso. Quantum. 2025;9:1679.

Article  Google Scholar 

Fawcett T. An introduction to ROC analysis. Pattern Recognit Lett. 2006;27(8):861–74.

Article  Google Scholar 

Ye F, Wang T, Yin H, et al. Development and validation of a nomogram to predict the individual future stroke risk for adult patients with Moyamoya disease: a multicenter retrospective cohort study in China. Front Neurol. 2021;12:669025.

Article  PubMed  PubMed Central  Google Scholar 

Becht E, Giraldo NA, Lacroix L, et al. Estimating the population abundance of tissue-infiltrating immune and stromal cell populations using gene expression. Genome Biol. 2016;17:218.

Article  PubMed  PubMed Central  Google Scholar 

Huang D, Wang Y, Lv J, et al. Proteomic profiling analysis of postmenopausal osteoporosis and osteopenia identifies potential proteins associated with low bone mineral density. PeerJ. 2020;8:e9009.

Article  PubMed  PubMed Central  Google Scholar 

Gold DT. Medication adherence: a challenge for patients with postmenopausal osteoporosis and other chronic illnesses. J Manag Care Pharm. 2006;12(6):S20–5.

PubMed  PubMed Central  Google Scholar 

Adler RA. Osteoporosis in men: a review. Bone Res. 2014;2:14001.

Article  PubMed  PubMed Central  Google Scholar 

Cao G, Hu S, Ning Y, et al. Traditional Chinese medicine in osteoporosis: from pathogenesis to potential activity. Front Pharmacol. 2024;15:1370900.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang Y, Guo X, Sun K, et al. Exploring the classification and treatment of osteoporosis from the perspectives of natural medicines, molecular targets, and symptom clusters. Sci Rep. 2025;15(1):10218.

Comments (0)

No login
gif