Non Polar Fraction from Selectively Inhibit Hep-2 C Cancer Cell Proliferation; Insights from GCMS and HRLCMS-QTOFS Profiling

Chaachouay N, Zidane L. Plant-derived natural products: a source for drug discovery and development. Drugs Drug Candidates. 2024;3(1):184–207.

Article  Google Scholar 

Payyappallimana U. Role of traditional medicine in primary health care: an overview of perspectives and challenging. 横浜国際社会科学研究= Yokohama J Soc Sci. 2010;14(6):57–77.

Martemucci G, Costagliola C, Mariano M, D’andrea L, Napolitano P, D’Alessandro AG. Free radical properties, source and targets, antioxidant consumption and health. Oxygen. 2022;2(2):48–78.

Article  CAS  Google Scholar 

Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, Sharifi-Rad J. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem. 2023;11:1158198.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Reddy VP. Oxidative stress in health and disease. Biomedicines. 2023;11(11):2925.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jomova K, Raptova R, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, Valko M. Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging. Arch Toxicol. 2023;97(10):2499–574.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Duletić-Laušević S. A study of phytochemistry, genoprotective activity, and antitumor effects of extracts of the selected Lamiaceae species. Plants. 2021;10(11):2306.

Article  PubMed  PubMed Central  Google Scholar 

Sen S, Chakraborty R, Sridhar C, Reddy YSR, De B. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. Int J Pharm Sci Rev Res. 2010;3(1):91–100.

CAS  Google Scholar 

Alper M, Özay C. Antioxidant activity and phenolic composition of ethanol extracts of Momordica charantia and Datura stramonium. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa. Dergisi. 2022;25(1):1–9.

Article  Google Scholar 

Michalak M. Plant extracts as skin care and therapeutic agents. Int J Mol Sci. 2023;24(20):15444.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Szymanska R, Pospisil P, Kruk J. (2016). Plant-derived antioxidants in disease prevention. Oxid Med Cell Longev. 2016:1920208.

Roy A (Ed.). Harnessing medicinal plants in cancer prevention and treatment. IGI Global. 2024.

Sun W, Shahrajabian MH. Therapeutic potential of phenolic compounds in medicinal plants—Natural health products for human health. Molecules. 2023;28(4):1845.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chalotra R, Dhanawat M, Maqbool M, Lamba N, Bibi A, Gupta S. Phytochemistry and pharmacology of Iris kashmiriana. Pharmacogn Res. 2022;14(4):350–5.

Article  CAS  Google Scholar 

Khan A, Tak H, Nazir R, Lone BA. In vitro and in vivo anthelmintic activities of Iris kashmiriana Linn. J Saudi Soc Agric Sci. 2018;17(3):235–40.

Article  Google Scholar 

Mir AH. Pharmacological and phytochemical properties of Iris kashmiriana baker as a potential medicinal plant of Kashmir Himalaya. Global J Res Med Plants Indigenous Med. 2014;3(12):460.

Google Scholar 

Bitwell C, Indra SS, Luke C, Kakoma MK. A review of modern and conventional extraction techniques and their applications for extracting phytochemicals from plants. Sci Afr. 2023;19:e01585.

CAS  Google Scholar 

Wong-Paz JE, Contreras-Esquivel JC, Rodríguez-Herrera R, Carrillo-Inungaray ML, López LI, Nevárez-Moorillón GV, Aguilar CN. Total phenolic content, in vitro antioxidant activity and chemical composition of plant extracts from semiarid Mexican region. Asian Pac J Trop Med. 2015;8(2):104–11.

Article  CAS  PubMed  Google Scholar 

Shraim AM, Ahmed TA, Rahman MM, Hijji YM. Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. Lwt. 2021;150:111932.

Article  CAS  Google Scholar 

Brand-Williams W, Cuvelier ME, Berset CLWT. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995;28(1):25–30.

Article  CAS  Google Scholar 

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–7.

Article  CAS  PubMed  Google Scholar 

Sánchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int. 2002;8(3):121–37.

Article  Google Scholar 

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–41.

Article  CAS  PubMed  Google Scholar 

Kumar P, Nagarajan A, Uchil PD. Analysis of cell viability by the MTT assay. Cold Spring Harbor Protocols. 2018;(6):pdb-prot095505.

Ahmad SS, Garg C, Kaur S. Metabolite profiling and cytotoxicity of Tragopogon dubius fractions against MCF-7 cells. Biochem Syst Ecol. 2025;123:105065.

Article  CAS  Google Scholar 

Liang CC, Park AY, Guan JL. In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007;2(2):329–33.

Article  CAS  PubMed  Google Scholar 

Chouhan M, Kumar S, Ahmad SS, Aataya FS, Garg C, Kaur S. Medicinal potential of Morchella esculenta oil inhibits cancer cell proliferation, metabolite characterization through GCMS, GC× GC–TOF–MS and UHPLC–QTOF–MS. Sci Rep. 2025;15(1):33311.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175(1):184–91.

Article  CAS  PubMed  Google Scholar 

Hussain M, Ahmed S, Ibrahim M, Khazir J, Ahmad SS, Thakur RK, Mir BA. Phenolic content, antioxidant and allelopathic potential of Artemisia brevifolia Wall. ex Dc. across the elevations of Western Himalayan region of Ladakh. South Afr J Bot. 2023;157:508–19.

Article  CAS  Google Scholar 

Palaiogiannis D, Chatzimitakos T, Athanasiadis V, Bozinou E, Makris DP, Lalas SI. Successive solvent extraction of polyphenols and flavonoids from Cistus creticus L. leaves. Oxygen. 2023;3(3):274–86.

Article  CAS  Google Scholar 

Boeing JS, Barizão ÉO, Silva e, Montanher BC, de Cinque Almeida PF, V., Visentainer JV. Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chem Cent J. 2014;8(1):48.

Article  PubMed  PubMed Central  Google Scholar 

Shi L, Zhao W, Yang Z, Subbiah V, Suleria HAR. Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environ Sci Pollut Res. 2022;29(54):81112–29.

Article  CAS  Google Scholar 

Huyut Z, Beydemir Ş, Gülçin İ. Antioxidant and antiradical properties of selected flavonoids and phenolic compounds. Biochem Res Int. 2017;2017(1):7616791.

PubMed  PubMed Central  Google Scholar 

Martínez S, Fuentes C, Carballo J. Antioxidant activity, total phenolic content and total flavonoid content in sweet chestnut (Castanea sativa Mill.) cultivars grown in Northwest Spain under different environmental conditions. Foods. 2022;11(21):3519.

Article  PubMed  PubMed Central  Google Scholar 

da Costa Cordeiro BMP, de Lima Santos ND, Ferreira MRA, de Araújo LCC, ARC Junior, da Conceição Santos AD, Paiva PMG. Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-candida agent by causing mitochondrial and lysosomal damages. BMC Complement Altern Med. 2018;18(1):284.

Johari MA, Khong HY. Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Adv Pharmacol Pharm Sci. 2019;2019(1):7428593.

Google Scholar 

Perez Gutierrez RM, Cotera F, L. B., Gonzalez AMN. Evaluation of the antioxidant and anti-glication effects of the hexane extract from Piper auritum leaves in vitro and beneficial activity on oxidative stress and advanced glycation end-product-mediated renal injury in streptozotocin-treated diabetic rats. Molecules. 2012;17(10):11897–919.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Talib WH, Alsayed AR, Barakat M, Abu-Taha MI, Mahmod AI. Targeting drug chemo-resistance in cancer using natural products. Biomedicines. 2021;9(10):1353.

Article  CAS 

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