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Establishing a cutting-edge protoplast technology platform for applying new genomic techniques in Cichorium spp
Genome editing technologies, especially those based on the CRISPR/Cas9 system, have revolutionized crop breeding by enabli...
Engineering enhanced signal peptides: A high-throughput computational pipeline for optimizing therapeutic protein production in CHO cells
Rational design of signal peptides (SPs), crucial for efficient therapeutic protein secretion in Chinese hamster ovary (CH...
Comparative transcriptomics of CAZy enzymes in white- and brown-rot agaricomycetes: Evolutionary insights into lignocellulose degradation and the relevance of GH16 glycoside hydrolase functional divergence
White-rot and brown-rot fungi (WRF and BRF, respectively) decompose lignocellulose, the main structural component of plant...
A human genome wide screen identifies miRNAs to increase recombinant Adeno-associated virus production in HEK293 cells
AAVAdeno-associated virusBAK1Bcl-2 Homologous Antagonist/KillerBAXApoptosis Regulator BAXCFLARCASP8 and FADD-Like Apoptosi...
Microbially induced carbonate precipitation as an innovative technology for achieves efficient Sr2+ bioremediation
Microbes have demonstrated remarkable potential for Sr2+ bioremediation under both laboratory and in situ conditions. Howe...
Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation
This study introduces a novel approach to optimizing the cultivation of Limnospira fusiformis through a hybrid Integrated ...
Impact of the compensation effect on the production of recombinant CD81-functionalized HIV-1 Gag virus-like particles and extracellular vesicles in HEK293 cells
CD81 is a membrane protein of the tetraspanin family present in the tetraspanin-enriched microdomains (TEMs). CD81 is inco...
Monoclonal antibodies production in microbial systems: Current status, challenges and perspectives
Monoclonal antibodies (mAbs) serve as indispensable tools in diagnostics, clinical therapeutics, and biomedical research. ...
Biocrystallization: Harnessing natural scaffolds for stable protein crystal formulations
There is a growing interest in developing precisely engineered and reliable crystalline formulations of proteins for medic...
Effect of culture medium composition and reuse on the growth of Porphyridium cruentum
Volume 90, 25 December 2025, Pages 65-76Author links open overlay panel, , , , AbstractThe aim of this study was to develo...
Proximity-based site-specific labeling of a native IgG Fab fragment by a fusion microbial transglutaminase-protein G variant
Volume 90, 25 December 2025, Pages 56-64Author links open overlay panel, , , , Highlights•A new enzymatic proximity-based ...
How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis
Volume 90, 25 December 2025, Pages 122-133Author links open overlay panel, , , Highlights•Food residues as a nutrient-rich...
Site-specifically immobilized D-amino acid dehydrogenase for the synthesis of D-phenylalanine
Aromatic d-amino acids (d-AAs) have gained increasing attention as chiral building blocks, with biocatalytic procedures em...
Recombinant production of silk fibroin in Nicotiana benthamiana using a modular library for transgene assembly.
Volume 90, 25 December 2025, Pages 313-326Author links open overlay panel, , , , Highlights•Developed a versatile modular ...
Mechanical properties and water absorption of shiitake mushroom-based films with natural rubber latex
Mechanical properties and water absorption were determined of films produced from powdered Lentinula edodes (shiitake) mus...
Exploring protein secretion-modulating and structural features of pro-peptides from S8 class subtilisin-like serine proteases in Bacillus subtilis
One of the most common post-translational modifications occurring in bacteria is proteolytic cleavage. Particularly, in se...
Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications
Automated protein purification is essential for meeting the growing demands of high-throughput sample processing capabilit...
From wheat bran to 4-vinylguaiacol: A green bioprocess featuring in situ product recovery
Valorizing agro-industrial byproducts such as wheat bran (WB) through microbial and enzymatic processing offers a sustaina...
Catalytic versatility of lipoxygenase from Microcystis aeruginosa
Lipoxygenases (LOXs) catalyze the regio- and enantioselective addition of molecular oxygen to polyunsaturated fatty acids ...
Investigating the dynamics of precursor availability for sesquiterpenoid production in the cytoplasm and plastid of Chlamydomonas reinhardtii
The green alga Chlamydomonas reinhardtii is an interesting organism for production of heterologous isoprenoid natural prod...
Screening assay for polyester hydrolyzing microorganisms using fluorescence-labelled poly(butylene adipate)
Available online 5 April 2025Author links open overlay panel, , , , Highlights•A novel method for detecting polymer hydrol...
A Distinct Autofluorescence Distribution Pattern Marks Enzymatic Deconstruction of Plant Cell Wall
Achieving an economically viable transformation of plant cell walls into bioproducts requires a comprehensive understandin...
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b
Volume 87, 25 July 2025, Pages 12-19Author links open overlay panel, , , Highlights•Type II methanotroph, M. trichosporium...
Microfluidic method for rapidly determining the protein and lipid yield of microalgae
Microalgae are a promising source of green energy. They produce valuable bioproducts, such as proteins and lipids, and rem...
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
This work studies, for the first time, the potential of a laccase from the endophyte fungus Hormonema sp. CECT-13092, comp...
Enzyme-assisted extraction and product profiling of fucosylated xyloglucan from industrial citrus pectin residues
Industrial pectin production from citrus peels involves extended, multi-step acidic extraction at elevated temperatures. P...
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment
Volume 87, 25 July 2025, Pages 105-111Author links open overlay panel, , , , , Highlights•Revolutionary approach: OncoFlow...
From the lab to the field and closer to the market: Production of the biopolymer cyanophycin in plants
A range of studies has investigated the production of biopolymers in plants but a comprehensive assessment of feasibility ...
Microalgal biorefineries in sustainable biofuel production and other high-value products
Microalgae has been emerging as a promising solution against the backdrop of the global need for sustainable, eco-friendly...
Antibacterial and biodegradable PLA-based nanofibers loaded with natural phenolic monoterpenes for sustainable biomedical or food application
Volume 87, 25 July 2025, Pages 1-11Author links open overlay panel, , , , , , , , AbstractAntibacterial biodegradable PLA-...
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