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Establishment and application of six-plex fluorescent quantitative PCR assay for the detection of calf diarrhea pathogens
Calf diarrhea is one of the most common diseases in calves, characterized by loose, soft or watery stools, dehydration and...
Real time PCR-based evaluation of live attenuated lumpy skin disease virus vaccines for immunogenicity and efficacy
Lumpy skin disease (LSD) is an important viral disease of cattle characterized by fever, multiple cutaneous nodules and pl...
Targeting Yellow-Fever Virus: Development of a specific aptamer to NS1 protein
Yellow Fever Virus (YFV), a mosquito-borne flavivirus, continues to pose a significant public health threat in tropical an...
Evaluation of two commercial multiplex RT-PCR tests for broad genotypic detection of viral gastroenteritis
Acute gastroenteritis (AGE) still remains one of the most common diseases in humans worldwide, being responsible for about...
Development of sensitive and specific indirect ELISA tests for the detection of antibodies against bovine leukemia virus in serum and milk samples
Bovine leukemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus (Hossain et al., 2025), which belongs to t...
Development of a rapid, real-time RT-RAA assay for sensitive detection of bovine enterovirus in fecal samples
Bovine enterovirus (BEV), a single-stranded RNA virus belonging to the genus Enterovirus in the Picornaviridae family, has...
Search and prove of efficient inhibitors against papain-like protease from SARS-CoV-2
The COVID-19, caused by the SARS-CoV-2 virus, was first reported in December 2019 (Huang et al., 2020) and rapidly spread ...
Development of end-point and quantitative polymerase chain reaction assays to detect and discriminate between blueberry scorch virus and blueberry virus S
Blueberry scorch virus (BlScV) and blueberry virus S (BVS) are closely related carlaviruses with the latter being only rec...
Rapid establishment and validation of a PMAxx-RT-qPCR method for infectious titer detection of freeze-dried live attenuated hepatitis A vaccine (H2 Strain)
Hepatitis A is an acute self-limiting disease caused by the HAV, primarily affecting young children(Gholizadeh et al., 202...
Application of digital PCR and CRISPR/Cas13a-based fluorescent assay for accurate and on-site detection of cotton leafroll dwarf virus
Cotton (Gossypium spp.) is a vital global crop, essential for both fiber and oil production, contributing significantly to...
Evaluation of dried blood spot testing for serological monitoring of epizootic and zoonotic pathogens in domestic pigs
Dried blood spots (DBS) constitute a stable, cost-efficient sampling matrix that can be collected in a minimally invasive ...
Evaluation of Tasso T20 to collect dried blood for the quantification of HIV-1 RNA
Quantification of HIV-1 RNA is important in monitoring disease progression and response to antiretroviral therapy in indiv...
Alternatives to central nervous system samples for enhanced rabies surveillance: Exploring natural infections of Lyssavirus rabies in striped skunks (Mephitis mephitis) in Northern Colorado
Enhanced rabies surveillance (ERS) of wildlife populations complements passive public health surveillance through active f...
Corrigendum to ‘optimization and proficiency testing of a pseudovirus-based assay for detection of HIV-1 neutralizing antibody in China’[journal of virological methods 185 (2012) 267– 275]
Volume 341, April 2026, 115319Author links open overlay panelJianhui Nie a f, Wenbo Wang a, Zhiheng Wen a, Aijing Song a, ...
Corrigendum to “An alternative model for HEV infection in the HepaRG cell line” [J. Virol. Methods 340 (2026) 115307]
Volume 341, April 2026, 115334Author links open overlay panelStacy Gellenoncourt a, Marie Pellerin a, Aïlona Marcadet-Haus...
Animal models illuminate dengue immunopathogenesis to guide vaccine and therapeutic developments
Dengue hemorrhagic fever is a severe health issue in many regions worldwide, caused by the dengue virus (DENV), which is p...
Genetic influence of IFN-γ gene polymorphisms on hepatitis C progression and recovery
Hepatitis C is a chronic liver disease of major public health concern, caused by Hepatitis C virus (HCV) (Tariq et al., 20...
Development and application of a real-time RT-PCR assay for the specific detection of influenza D virus
The D type influenza virus (Influenza D virus, IDV), a novel member of the influenza virus family, belongs to the family O...
Prevalence and ribavirin-mediated elimination of Morchella importuna endornavirus 1 (MiEV1) in cultivated Morchella species in China
Mycoviruses are widely distributed across major fungal taxa, including yeasts, edible mushrooms, and pathogenic fungi (Zha...
Development of an ELISA using recombinant Dengue NS1 constructs to measure antibody titers and predict dengue disease outcome in a pediatric population in India
Dengue is a major global health burden caused by infection with one of the four dengue virus (DENV) serotypes, which belon...
Development of an ORF154-DIVA ELISA for Distinguishing LSDV-Infected and Vaccinated Animals
Lumpy Skin Disease (LSD) is characterized by fever, swollen lymph nodes, and the formation of firm, round skin nodules tha...
Development and Validation of Novel Cell-free Direct Neutralization Assay for SARS-CoV-2
Neutralizing antibody titer elicited through infection or vaccination is widely accepted as a reliable surrogate for prote...
Corrigendum to “Rapid and sensitive detection of shrimp infectious myonecrosis virus using a reverse transcription loop-mediated isothermal amplification and visual colorogenic nanogold hybridization probe assay” [J. Virol. Methods, Vol. 193 (2013) 542–547]
Available online 10 June 2025, 115199Author links open overlay panelNarong Arunrut a b, Jantana Kampeera a, Rungkarn Suebs...
Establishment of an immunoperoxidase monolayer assay for the detection of African swine fever virus antibodies
African swine fever (ASF) is an acute, hemorrhagic, highly contagious, infectious disease of domestic and wild pigs, cause...
Technical validation of a multiplex real-time PCR for combined detection of Rift Valley fever, chikungunya, Zika and dengue viruses
Several arthropod-borne (arbo)-viruses have overlapping symptoms, insect vectors and geographical occurrence. With little ...
SHERLOCK, a novel CRISPR-Cas13a-based assay for detection of infectious bursal disease virus
Infectious bursal disease virus (IBDV) is the pathogen responsible for infectious bursal disease (IBD), also known as Gumb...
Development and clinical evaluation of a MPXV antigen-detecting rapid diagnostic test
Cookies are used by this site. Cookie SettingsAll content on this site: Copyright © 2025 Elsevier B.V., its licensors, an...
Room temperature storage and shipping of encapsulated synthetic RNAs as quality control materials for SARS-CoV-2 molecular diagnostic assays
The Coronavirus pandemic has led to an unprecedented need for diagnostic tests to rapidly detect the presence of this path...
Optimization of human T-cell lymphotropic virus type 1 (HTLV-1) serological and molecular diagnosis for alternative blood samples
Human T-cell Lymphotropic Virus Type 1 (HTLV-1) is a human retrovirus that causes mainly asymptomatic chronic asymptomatic...
Comparative performance of serum and plasma samples in SARS-CoV-2 serology and neutralization assays
The SARS-CoV-2 pandemic catalyzed the rapid development and deployment of serological assays, which have been pivotal for ...
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